Trypsin-catalysed transpeptidations
نویسندگان
چکیده
منابع مشابه
Optical and chemical identification of kinetic steps in trypsin- and chymotrypsin-catalysed reactions.
Previous interpretations of the mechanism of trypsin- and chymotrypsin-catalysed reactions in terms of two intermediates, the Michaelis complex and an acyl-enzyme, were based on steady-state studies and on the observation of individual steps under sub-optimum conditions. In the present paper new methods for the rapid analysis of chemical events and for the spectrophotometric detection of indivi...
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The heats of reaction of trypsin with soybean (Kunitz), ovomucoid, and lima bean inhibitors have been measured at pH 5.0 and 10’ and 25”. All the reactions were endothermic, with AH ranging from 8.6 Cal per mole for the lima bean inhibitor to 15.3 Cal per mole for the soybean inhibitor. Equilibrium constants for the association were calculated from the dependence of residual tryptic activity up...
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From the ‡European Molecular Biology Laboratory (EMBL) Hamburg c/o DESY, D-22607 Hamburg, Germany, the **Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61–704 Poznan, Poland and Institute of Biochemistry and Molecular Biology, University Hospital, Hamburg-Eppendorf, c⁄o DESY, 22603 Hamburg, Germany, the ¶Laboratoire de Cristallographie et Modelisation des Matériaux Mineraux et B...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1954
ISSN: 0306-3283
DOI: 10.1042/bj0570529